Significance of additional RFLP single locus probes analysis after STR based determination of sibship

Autosomal STR typing alone seems to be no sufficient tool for resolving deficiency cases (e.g. cases of questioned paternity or half-sibships). Therefore, we investigated whether the additional analysis of RFLP single locus probes can improve the solution of such complicated kinship cases. We analyzed 207 children and men from 101 families using the AmpFlSTRIdentifiler multiplex PCR kit and three RFLP single locus probes. A comparison between each child and all unrelated men resulted in 11,023 man / child pairs. Less than three excluding STRs were found in 125 child / unrelated man pairs (1.13%). Additional analysis of RFLP results reduced the number of ambiguous cases to 35. Half-sibling pairs were simulated using STR results from 20 cases with high paternity probabilities (group 1) and relatively low paternity probabilities (group 2). Using a commercially available computer program we calculated probabilities for 778 half-sibling pairs. In 35 pairs (4.49%) half-sibling probabilities over 90.0% could be calculated. Additional investigation of RFLP single locus probes did not lead to a more reliable evaluation of these results. The combined investigation of autosomal STRs and RFLP single locus probes can satisfactorily solve deficient paternities but does not contribute to the solution of questioned half-sibships.     

Franco-German Intelligence Cooperation and the Internationalization of Algeria's War of Independence (1954–62)

Between 1958 and 1960, the French domestic security and intelligence services came to establish a close, multi-layered, and secret working relationship with their German counterparts. The purpose of this collaborative arrangement was to enlist German support in combating the subversive activities of the Algerian Front de Libération Nationale, whose members had taken refuge in Germany. In particular, the metropolitan authorities sought to impose on their German counterparts some of the same methods of colonial policing and intelligence that characterized their own counter-insurgency in France. These efforts proved counter-productive, however, for in internationalizing the Algerian war, they drew public attention to the colonial nature of France's hold over Algeria.     

Religion and inter-ethnic violence in Indonesia

Commentators have long struggled to understand state-society relations in Asia within the framework of the dominant liberal-democratic conceptualisation of civil society. This article examines the relevance of Antonio Gramsci's theory of civil society for understanding contemporary Cambodia and Vietnam, with reference to both legal and social frameworks. Such an analysis illuminates important aspects of state-society relations in Southeast Asia that tend to be overlooked by dominant liberal and Marxist perspectives. This article argues, however, that the utility of Gramsci's conception of civil society for understanding state-society relations in Cambodia and Vietnam, by retaining the notion of civil society as a realm associatively separate from the state, is limited.     

The Impact of Drinking Water Quality and Sanitation on Child Health: Evidence from Rural Ethiopia

This paper examines the association between stored drinking water quality and sanitation on diarrhoea incidence among under-five children in Ethiopia. Using primary household survey data and microbiological water quality testing for Escherichia coli, our results show that uncontaminated stored drinking water and safe child stool disposal are associated with decreased child diarrhoea incidences of 18 and 20 percentage points, respectively. In contrast, neighbourhood concentration of pit latrine shows an increased incidence of child diarrhoea of 16 percentage points. To protect rural children from the risk of contracting diarrhoea, improving household drinking water quality and changing people’s behaviour towards safe sanitation practices is needed.     

The decision to legalize gambling: a model of why states do what they do

This article compares the three major forms of legalized gambling currently being utilized by states as revenue measures. Unlike most of the previous gambling research, it focuses upon the choice of forms, rather than only the decision to adopt gambling measures. It compares forms of gambling using a variation on a previously published schema of evaluative criteria for revenue types and presents a series of models of why states choose to legalize certain types of gambling. The results suggest that iotteries and parimutuels are similar choices for revenue raising, since both are likely to be adopted in states which are liberal, which exhibit somewhat weak interest groups, and which have relatively low Protestant fundamentalist church membership. Casinos, however, appear to conform to a different decision model and are less likely to be operated in states with large numbers of older citizens and in states which do not already have other forms of gambling.     

Claims to legitimacy count: Why sanctions fail to instigate democratisation in authoritarian regimes

International sanctions are one of the most commonly used tools to instigate democratisation in the post‐Cold War era. However, despite long‐term sanction pressure by the European Union, the United States and/or the United Nations, non‐democratic rule has proven to be extremely persistent. Which domestic and international factors account for the regimes' ability to resist external pressure? Based on a new global dataset on sanctions from 1990 to 2011, the results of a fuzzy set qualitative comparative analysis (fsQCA) provide new insights for the research on sanctions and on authoritarian regimes. Most significantly, sanctions strengthen authoritarian rule if the regime manages to incorporate their existence into its legitimation strategy. Such an unintended ‘rally‐round‐the‐flag’ effect occurs where sanctions are imposed on regimes that possess strong claims to legitimacy and have only limited economic and societal linkages to the sender of sanctions.     

Forensic imaging of projectiles using cone-beam computed tomography

In patients with gunshot injuries, it is easy to detect a projectile within the body due to the high-density of the object, but artefacts make it difficult to obtain information about the deformation and the exact location of the projectile in surrounding tissues. Cone-beam computed tomography (CBCT) is a new radiological imaging modality that allows radio-opaque objects to be localised and assessed in three dimensions. The full potential of the use of CBCT in forensic medicine has not yet been explored.In this study, three different modern projectiles were fired into the heads of pig cadavers (n = 6) under standardised conditions. Tissue destruction and the location of the projectiles were analysed separately using CBCT and multi-slice computed tomography (MDCT).The projectiles had the same kinetic energy but showed considerable differences in deformation behaviour. Within the study groups, tissue destruction was reproducible. CBCT is less severely affected by metallic artefacts than MDCT. Therefore CBCT is superior in visualising bone destruction in the immediate vicinity of the projectile and projectile deformation, whereas MDCT allows soft tissue to be evaluated in more detail.CBCT is an improved diagnostic tool for the evaluation of gunshot injuries. In particular, it is superior to MDCT in detecting structural hard-tissue damage in the immediate vicinity of high-density metal projectiles and in identifying the precise location of a projectile in the body.     

The problem of DNA contamination in forensic case work—How to get rid of unwanted DNA?

The PCR technique has become a powerful and very sensitive tool in a broad field of research, that is, molecular biology, medical diagnostics, population genetics, ancient DNA analysis and forensic casework.However, the high sensitivity down to single molecules can easily cause false-positive PCR results due to different types of contamination. In this study, artificial DNA contaminations (saliva and pure DNA) were treated with UV irradiation and other decontamination procedures. A satisfactory DNA removal could not be achieved, emphasizing the necessity of contamination avoidance.